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Synthesis of Indoxyl-glycosides for Detection of Glycosidase Activities
Published on: May 27, 2015
Purification and partial characterization of glyoxalase I from a higher plant Brassica juncea
1Plant Research Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
FEBS Letters
|May 6, 1991
Abstract:
The glyoxalase I was purified from Brassica juncea by affinity chromatography on S-hexyl GSH sepharose 4B. Homogeneity of the protein was confirmed electrophoretically by a silver stained gel. Activity staining on a native starch gel also showed a single band. The effect of glutathione, methylglyoxal, and pH on enzyme kinetics was studied. Magnesium was found to stimulate the enzyme activity.
