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Mutations in the rpsL gene are involved in streptomycin resistance in Campylobacter coli

Satu Olkkola1, Pekka Juntunen, Helmi Heiska

  • 1Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland. satu.olkkola@helsinki.fi

Microbial Drug Resistance (Larchmont, N.Y.)
|April 8, 2010
PubMed

Insights

Streptomycin resistance in Campylobacter coli is primarily linked to mutations in the rpsL gene. This study found no direct association between streptomycin and erythromycin resistance mechanisms in this bacterium.

Area of Science:

  • Microbiology
  • Bacterial Genetics
  • Antimicrobial Resistance

Background:

  • Campylobacter coli is a significant foodborne pathogen.
  • Understanding streptomycin resistance mechanisms is crucial for effective treatment.
  • The interplay between different antibiotic resistance mechanisms requires investigation.

Purpose of the Study:

  • To elucidate the genetic basis of streptomycin resistance in Campylobacter coli.
  • To investigate potential links between streptomycin and erythromycin resistance.
  • To identify specific genes and mutations conferring streptomycin resistance.

Main Methods:

  • Analysis of mutations in putative streptomycin resistance target genes (rpsL, rrs, gidB).
  • Evaluation of the aadE gene.
  • Experimental evolution of resistance to streptomycin and erythromycin.
  • Assessment of efflux pump inhibitor effects on streptomycin resistance.

Main Results:

  • Mutations in the rpsL gene were significantly associated with streptomycin resistance in C. coli.
  • In vitro-induced mutations were more diverse than in vivo mutations.
  • No resistance-associated mutations were found in rrs or gidB genes.
  • No aadE gene was detected in the isolates.
  • No cross-resistance or association between streptomycin and erythromycin resistance was observed.
  • The CmeABC efflux pump did not appear to influence streptomycin resistance.

Conclusions:

  • Streptomycin resistance in Campylobacter coli is predominantly mediated by mutations in the rpsL gene.
  • No clear link was established between streptomycin and erythromycin resistance mechanisms in the studied C. coli isolates.
  • Further research may be needed to explore other potential resistance determinants.

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