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Recombinant aequorin as a probe for cytosolic free Ca2+ in Escherichia coli
M R Knight1, A K Campbell, S M Smith
1Institute of Cell and Molecular Biology, University of Edinburgh, UK.
FEBS Letters
|May 6, 1991
Abstract:
We describe a novel and simple method for the measurement of bacterial cytosolic free calcium ([Ca2+]i) using recombinant aequorin reconstituted within live bacterial cells. Using this method we have measured the effects of external calcium, complement, phagocytosis and antibiotics on the [Ca2+]i of Escherichia coli. In principle this method should be applicable to any genetically transformable organism and should suffer fewer problems than fluorescent dyes for subcellular calcium measurement.