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Updated: Jun 14, 2026

Quantifying Yersinia pseudotuberculosis Type III Secretion System Activity Following Iron Starvation and Anaerobic Growth
Published on: May 31, 2024
Characterization of an aspartate-dependent acid survival system in Yersinia pseudotuberculosis
Yangbo Hu1, Pei Lu, Yong Zhang
1State Key Laboratory of Virology, Wuhan Institute of Virology, The Chinese Academy of Sciences, Wuhan, China.
Abstract:
Enteric bacteria have developed various survival systems that protect against acid stress. In this study, an aspartate-dependent acid survival system is characterized in Yersinia pseudotuberculosis. The expression of aspartase (AspA) was confirmed to be increased at acidic pH by proteomic and lacZ fusion analyses. Addition of aspartate increased acid survival of the wild type but not the aspA knockout mutant. AspA increases acid survival by producing ammonia as demonstrated by mutation and in vitro enzyme activity analyses. This is the first demonstration that an enzyme involved in aspartate metabolism plays a role in acid survival in an enteric bacterium.
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