Related Experiment Video
Updated: Jun 14, 2026
08:11
Characterization of Cell Membrane Extensions and Studying Their Roles in Cancer Cell Adhesion Dynamics
Published on: March 26, 2018
Adhesive interactions regulate transcriptional diversity in malignant B cells
Liat Nadav-Dagan1, Tal Shay, Nili Dezorella
1Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Molecular Cancer Research : MCR
|April 8, 2010
Summary
Cell adhesion to fibronectin in B-cell malignancies impacts gene expression, potentially driving multiple myeloma (MM) development. Adhesion hinders B-cell differentiation, upregulating MM-associated genes.
Area of Science:
- Hematology
- Oncology
- Molecular Biology
Background:
- Genetic profiling of B-cell malignancies is crucial for understanding disease progression and outcomes.
- The interplay between intrinsic gene expression and microenvironmental factors in B-cell malignancies remains unclear.
Purpose of the Study:
- To investigate how adhesive interactions regulate gene expression programs in B-cell malignancies.
- To determine the role of fibronectin adhesion in B-cell differentiation and the expression of multiple myeloma (MM)-associated genes.
Main Methods:
- Utilized ARH-77 human malignant B-cell variants with differential fibronectin adhesion properties.
- Compared gene expression profiles of adherent (Type A) and non-adherent (Type F) cells, as well as fibronectin-deprived adherent cells (AF cells).
- Employed bioinformatic analyses to correlate cell adhesion with differentiation state and MM-associated gene expression.
Main Results:
- A strong correlation was observed between cell adhesion to fibronectin and B-cell differentiation status.
- Highly adherent Type A cells exhibited increased expression of NF-kappaB-regulated genes and MM-associated genes.
- Fibronectin adhesion stimulated the transcription of MM-related proto-oncogenes, while Type F cells showed gene expression linked to higher B-cell differentiation.
Conclusions:
- Cell adhesion to fibronectin attenuates B-cell differentiation in malignant B-cells.
- This adhesion-mediated process leads to the upregulation of specific genes implicated in the pathogenesis of multiple myeloma.
- Microenvironmental cues, such as fibronectin adhesion, significantly influence the molecular landscape of B-cell malignancies.
Related Concept Videos
01:19Cadherins in Tissue Organization
The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
Cell Sorting During Development
Cell sorting plays an...
02:23Abnormal Proliferation
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
01:20Cell Adhesion Molecules - Types and Functions
Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily involved in a...
CAM Families
The Integrin family of proteins is primarily involved in a...
01:37Epigenetic Regulation
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
X-chromosome...
01:46Epigenetic Regulation
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
01:17Intracellular Signaling Affects Focal Adhesions
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...