Enhancing biological phosphorus removal with glycerol.
Q Yuan1, R Sparling, P Lagasse
1Department of Civil Engineering, University of Manitoba, 15 Gillson Street E1-368, Winnipeg, Canada R3T 5V6. umyuan3@cc.umanitoba.ca
Summary
Glycerol can enhance biological phosphorus removal in wastewater treatment, but only when pre-fermented with waste activated sludge to produce volatile fatty acids. This pre-fermentation step is crucial for effective phosphorus removal using glycerol as a carbon source.
Area of Science:
- Environmental Science
- Environmental Engineering
- Biotechnology
Background:
- Enhanced biological phosphorus removal (EBPR) processes are vital for nutrient management in wastewater treatment.
- Acetate is a common external carbon source for EBPR, but its availability can be limited.
- Glycerol, a byproduct of biodiesel production, is a potential alternative carbon source.
Purpose of the Study:
- To investigate the effectiveness of glycerol as an external carbon source for EBPR.
- To determine the optimal method for utilizing glycerol in EBPR systems.
- To enhance phosphorus removal efficiency in wastewater treatment.
Main Methods:
- Operated an enhanced biological phosphorus removal (EBPR) reactor with acetate as the carbon source.
- Substituted acetate with glycerol in the feed to assess EBPR performance.
- Fermented waste activated sludge (WAS) with glycerol in an off-line fermenter.
- Supplied the EBPR system with VFA-enriched supernatant from the WAS fermenter.
Main Results:
- EBPR operated successfully with acetate.
- EBPR process failed when glycerol was directly substituted for acetate.
- Fermentation of WAS with glycerol produced significant volatile fatty acids (VFA).
- Supplying VFA-enriched supernatant to the EBPR system enhanced phosphorus removal.
Conclusions:
- Direct addition of glycerol to EBPR is ineffective for phosphorus removal.
- Pre-fermentation of glycerol with waste activated sludge is essential for VFA production.
- VFA-enriched supernatant from glycerol fermentation effectively enhances EBPR.
- Fermenting glycerol with WAS is the recommended approach for using glycerol as a carbon source in EBPR.


