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A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target
Published on: May 10, 2022
Evaluation of inhibitory effects of thiobarbituric acid derivatives targeting HCV NS5B polymerase
Jong-Ho Lee1, Sangyoon Lee, Mi Young Park
1Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Yong-In 449-791, Korea.
Abstract:
A series of thiobarbituric acid derivatives were constructed and evaluated for inhibitory activity on hepatitis C virus NS5B polymerase. In biochemical assays using purified viral polymerase and RNA template, the IC(50) value was improved to 0.41 microM from the original compound's 1.7 microM value. In HCV subgenomic replicon assay, the EC(50) value was improved to 3.7 microM from the original compound's 12.3 microM value. CC(50) was higher than 77 microM for all compounds tested, suggesting that they are useful candidates for anti-HCV therapy.
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