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Related Concept Videos

Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
Flame Photometry: Overview01:02

Flame Photometry: Overview

Flame photometry, also known as flame emission spectrometry, is a technique used for the qualitative and quantitative analysis of elements present in a sample using a flame as the source of excitation energy. The concept of flame photometry was realized in the early 1860s by Kirchhoff and Bunsen, who discovered that specific elements emit characteristic radiation when excited in flames. The first instrument developed for this purpose was used to measure sodium (Na) in plant ash using a Bunsen...
Atomic Absorption Spectroscopy: Radiation and Light Sources01:13

Atomic Absorption Spectroscopy: Radiation and Light Sources

Atomic absorption spectroscopy (AAS) relies on the Beer-Lambert law, which requires that the radiation source emits a narrow range of wavelengths to match the absorption characteristics of the analyte atom. The primary criteria for choosing an appropriate radiation source in AAS is to provide a precise and intense emission at specific wavelengths that will allow accurate detection of the analyte.
Two common narrow-range 'line' sources used in AAS are hollow-cathode lamps (HCLs) and...
Atomic Absorption Spectroscopy: Interference01:25

Atomic Absorption Spectroscopy: Interference

Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
UV–Vis Spectroscopy: Beer–Lambert Law01:09

UV–Vis Spectroscopy: Beer–Lambert Law

The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann Heinrich Lambert and August Beer. Lambert's law states that when light passes through a medium, the loss in intensity is directly proportional to the original intensity and the path length of the light. Beer's law proposed that the transmittance of a solution remains constant if the product of concentration and path length is constant. The modern...

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Related Experiment Video

Updated: Jun 14, 2026

An Intra-Tissue Radiometry Microprobe for Measuring Radiance In Situ in Living Tissue
09:10

An Intra-Tissue Radiometry Microprobe for Measuring Radiance In Situ in Living Tissue

Published on: June 2, 2023

Light absorption measurements: new techniques.

G Hänel, R Busen, C Hillenbrand

    Applied Optics
    |April 8, 2010
    PubMed
    Summary

    A new radiometer simplifies solar wavelength radiation measurements. Aerosol particle absorption significantly increases atmospheric temperature, particularly in industrial areas.

    Area of Science:

    • Atmospheric Science
    • Radiometry
    • Aerosol Physics

    Background:

    • Accurate measurement of solar radiation is crucial for understanding atmospheric processes.
    • Aerosol particles play a significant role in Earth's energy budget by absorbing solar radiation.
    • Existing methods for measuring aerosol light absorption can be complex and time-consuming.

    Purpose of the Study:

    • To describe a new radiometer designed for simplified measurement of solar wavelength radiation.
    • To present two methods for quantifying radiant energy absorbed by aerosol particles: photometric and calorimetric.
    • To determine the atmospheric heating rate caused by aerosol light absorption.

    Main Methods:

    • Development and description of a novel radiometer for solar radiation measurement.

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  • Application of a photometric technique for analyzing aerosol particles collected on filters.
  • Utilization of a calorimetric technique for in situ measurement of radiant energy absorption by aerosols.
  • Main Results:

    • The new radiometer simplifies the measurement of solar radiation supply.
    • Both photometric and calorimetric methods effectively quantify aerosol light absorption.
    • Data indicate substantial atmospheric temperature increases attributable to aerosol absorption, especially in industrial regions.

    Conclusions:

    • The developed radiometer and absorption measurement techniques provide valuable data for atmospheric heating rate calculations.
    • Aerosol light absorption is a significant factor contributing to atmospheric warming.
    • Findings highlight the impact of industrial emissions on atmospheric temperature through aerosol absorption.