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Updated: Jun 14, 2026

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells
Published on: August 11, 2020
Improvement in resolution by nearly confocal microscopy
I J Cox1, C J Sheppard, T Wilson
1University of Oxford, Department of Engineering Science, Parks Road, Oxford OX1 3PJ, UK.
Abstract:
In a conventional confocal microscope the resolution is improved over that attainable in a conventional instrument. A further improvement in resolution is produced when the detector pinhole is offset resulting in nearly confocal operation. For the case where the pinhole is placed over the first dark ring in the Airy disk in the detector plane, dark-field conditions are produced by a very simple method.
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