Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Atomic Force Microscopy

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

Overview of Microscopy Techniques

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Hyperspectral imaging system based on a single-pixel camera design for detecting differences in tissue properties.

Applied optics·2018

Same author

Optical Fiber Sensing Using Quantum Dots.

Sensors (Basel, Switzerland)·2017

Same author

Spectroscopic characterization of oral epithelial dysplasia and squamous cell carcinoma using multiphoton autofluorescence micro-spectroscopy.

Lasers in surgery and medicine·2017

Same author

Robust sub-micrometer displacement measurement using dual wavelength speckle correlation.

Optics express·2015

Same author

Production and assessment of decellularized pig and human lung scaffolds.

Tissue engineering. Part A·2013

Same author

In vivo layer-resolved characterization of oral dysplasia via nonlinear optical micro-spectroscopy.

Biomedical optics express·2012

Search research articles

Related Experiment Video

Updated: Jun 14, 2026

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Published on: October 24, 2014

Hybrid shear force feedback/scanning quantitative phase microscopy applied to subsurface imaging.

Kert Edward¹, Faramarz Farahi, Robert Hocken

¹Department of Physics and Optical Science, University of North Carolina at Charlotte, Charlotte, NC, 28223, USA. kedward@uncc.edu

|April 8, 2010

Summary

This summary is machine-generated.

Quantitative phase microscopy can now reveal subsurface details in transparent biological samples. This new method combines phase and shear-force data to distinguish surface and internal structures.

More Related Videos

Active Probe Atomic Force Microscopy with Quattro-Parallel Cantilever Arrays for High-Throughput Large-Scale Sample Inspection

Active Probe Atomic Force Microscopy with Quattro-Parallel Cantilever Arrays for High-Throughput Large-Scale Sample Inspection

Published on: June 13, 2023

Related Experiment Videos

Last Updated: Jun 14, 2026

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Published on: October 24, 2014

Active Probe Atomic Force Microscopy with Quattro-Parallel Cantilever Arrays for High-Throughput Large-Scale Sample Inspection

Active Probe Atomic Force Microscopy with Quattro-Parallel Cantilever Arrays for High-Throughput Large-Scale Sample Inspection

Published on: June 13, 2023

Area of Science:

Biophysics
Optical Microscopy
Cell Biology

Background:

Quantitative phase microscopy (QPM) is vital for studying transparent biological specimens.
QPM data often intermingles surface and subsurface information, complicating analysis.
Decoupling these components is a significant challenge in microscopy.

Purpose of the Study:

To develop a straightforward method for extracting subsurface information from QPM data.
To differentiate between surface morphology and subsurface structures in biological samples.
To enhance the analytical capabilities of quantitative phase microscopy.

Main Methods:

Simultaneous acquisition of quantitative phase and shear-force feedback topography data.
Development of a novel procedure to decouple surface and subsurface information.
Application of the method to both fabricated test samples and biological specimens.

Main Results:

Successfully extracted subsurface sample information using the combined data approach.
Identified distinct subsurface features in fabricated samples.
Revealed subsurface structures within fish erythrocytes, demonstrating biological applicability.

Conclusions:

The presented procedure effectively separates surface and subsurface information in QPM.
This technique offers a simple yet powerful way to probe internal structures of transparent samples.
The findings advance the application of QPM in biological and materials science research.