Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic multimode fiber specklegram sensor with automated training data generation for bed-exit prediction.

Biomedical optics express·2026
Same author

Growth of an alumina overlayer deposited on a Au<sub>101</sub>/TiO<sub>2</sub> model catalyst <i>via</i> atomic layer deposition.

Nanoscale·2026
Same author

Development of a gut-on-a-chip platform to monitor dynamic GLP-1 secretion from primary intestinal tissue.

Biosensors & bioelectronics·2026
Same author

A Side-viewing Fluorescence Needle Probe for Brain Biopsy Guidance.

IEEE transactions on bio-medical engineering·2026
Same author

In-Line Tapered Microfiber Sensors for Label-Free Simultaneous Detection of Dual Genes via Enzymatic Recombinase Amplification.

ACS sensors·2026
Same author

A rigorous theoretical model of fluorescence-based fiber optic sensors: application to D-shaped fibers.

Scientific reports·2025

Related Experiment Video

Updated: Jun 14, 2026

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
10:21

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Exposed-core microstructured optical fibers for real-time fluorescence sensing.

Stephen C Warren-Smith1, Heike Ebendorff-Heidepriem, Tze Cheung Foo

  • 1Centre of Expertise in Photonics, Institute for Photonics & Advanced Sensing, The University of Adelaide, Adelaide, Australia. stephen.warrensmith@adelaide.edu.au

Optics Express
|April 8, 2010
PubMed
Summary

New exposed-core optical fibers offer faster sensing. These microstructured fibers enable real-time measurements by enhancing evanescent field interactions for improved sensor response times.

More Related Videos

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing
08:12

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

Published on: March 13, 2013

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
10:34

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

Published on: August 20, 2012

Related Experiment Videos

Last Updated: Jun 14, 2026

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
10:21

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing
08:12

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

Published on: March 13, 2013

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
10:34

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

Published on: August 20, 2012

Area of Science:

  • Materials Science
  • Optical Engineering
  • Sensor Technology

Background:

  • Microstructured optical fibers (MOFs) are advanced photonic devices.
  • Exposed-core designs offer unique evanescent field interaction capabilities for sensing applications.

Purpose of the Study:

  • To demonstrate novel fabrication methods for exposed-core microstructured optical fibers.
  • To evaluate the sensing performance of exposed-core fibers compared to enclosed-core fibers.

Main Methods:

  • Fabrication of glass microstructured optical fibers with a suspended, partially exposed core.
  • Experimental comparison of exposed-core and enclosed-core fibers as evanescent field sensors.

Main Results:

  • Successfully fabricated exposed-core microstructured optical fibers.
  • Exposed-core fibers demonstrated significantly improved measurement response times compared to enclosed-core fibers.
  • Small core size facilitates strong evanescent field interactions.

Conclusions:

  • Exposed-core microstructured optical fibers are effective for enhanced evanescent field sensing.
  • These fibers enable faster, real-time, and potentially distributed measurements.
  • The demonstrated fabrication methods open avenues for advanced optical sensing.