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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Related Experiment Video

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Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
10:21

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Optical-fiber-microsphere for remote fluorescence correlation spectroscopy.

Heykel Aouani1, Frédérique Deiss, Jérôme Wenger

  • 1Institut Fresnel, CNRS, Aix-Marseille Université, Ecole Centrale Marseille, Campus de St Jérôme, 13397 Marseille, France.

Optics Express
|April 8, 2010
PubMed
Summary
This summary is machine-generated.

This study demonstrates single-molecule detection using fiber-optic fluorescence correlation spectroscopy (FCS) enhanced by a latex microsphere and photonic nanojet effect. This breakthrough enables remote sensing applications by overcoming current sensitivity limitations.

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Area of Science:

  • Biophotonics
  • Spectroscopy
  • Nanotechnology

Background:

  • Fluorescence correlation spectroscopy (FCS) is valuable for remote optical-fiber sensors.
  • Current fiber-based FCS lacks sensitivity for single-molecule detection due to high background noise.
  • Existing methods require bulky microscope setups, limiting remote and in vivo applications.

Purpose of the Study:

  • To enhance the sensitivity of fiber-optic fluorescence correlation spectroscopy (FCS) for single-molecule detection.
  • To overcome limitations of high background and low sensitivity in current fiber-based FCS.
  • To enable remote and in vivo applications of FCS by reducing setup size.

Main Methods:

  • Utilized an optical fiber integrated with a latex microsphere for FCS analysis.
  • Leveraged the photonic nanojet effect to boost detection sensitivity.
  • Performed experiments to demonstrate single-molecule detection capabilities.

Main Results:

  • Achieved single-molecule level sensitivity in fiber-optic FCS.
  • Demonstrated the effectiveness of the latex microsphere and photonic nanojet effect.
  • Showcased a significant improvement in detection sensitivity compared to conventional methods.

Conclusions:

  • The developed fiber-optic FCS system with a latex microsphere achieves single-molecule sensitivity.
  • The photonic nanojet effect is crucial for enhancing sensitivity in this setup.
  • This approach offers a promising pathway for miniaturized, remote, and in vivo FCS applications.