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Related Experiment Video

Updated: Jun 14, 2026

Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications
05:31

Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications

Published on: December 21, 2014

Amnion epithelial cell isolation and characterization for clinical use.

Sean Murphy1, Sharina Rosli, Rutu Acharya

  • 1Department of Obstetrics and Gynaecology, Monash University, Clayton, Victoria, Australia.

Current Protocols in Stem Cell Biology
|April 8, 2010
PubMed
Summary

Human amnion epithelial cells (hAECs) offer a promising cell therapy source due to their differentiation potential and low immunogenicity. This study details an effective, animal-product-free method for isolating, culturing, and cryopreserving these versatile stem cells.

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Area of Science:

  • Stem Cell Biology
  • Regenerative Medicine
  • Cell Therapy

Background:

  • Human amnion epithelial cells (hAECs) possess stem cell markers and multilineage differentiation capacity.
  • hAECs exhibit low immunogenicity and potent immunosuppressive properties, suggesting therapeutic potential.
  • Existing cell preparation methods may not align with clinical use guidelines.

Purpose of the Study:

  • To describe an efficient, animal product-free method for hAEC isolation, culture, and cryopreservation.
  • To characterize hAECs prepared using this method for clinical applications.
  • To demonstrate the multilineage differentiation potential of these hAECs.

Main Methods:

  • Isolation and culture of hAECs using an animal product-free protocol.

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Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
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Last Updated: Jun 14, 2026

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In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
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Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

  • Characterization of cells post-culture, including karyotype, cell cycle, and telomere length analysis.
  • Assessment of differentiation into endoderm, mesoderm, and ectoderm lineages via PCR, immunocytochemistry, and histology.

    • Main Results:

    • An efficient and effective animal product-free method for hAEC preparation was established.
    • Characterization confirmed cell stability and safety markers (karyotype, cell cycle, telomere length).
    • hAECs demonstrated robust differentiation into all three primary germ layers, confirmed by lineage-specific markers.

    Conclusions:

    • The described animal product-free method yields clinically relevant hAECs.
    • hAECs are a viable cell source for regenerative medicine due to their differentiation capacity and immunomodulatory properties.
    • This protocol supports the clinical translation of hAEC-based therapies.