Detection of prostate-specific antigen with a paired surface plasma wave biosensor

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Nanotechnology

Background:

• Prostate-specific antigen (PSA) is a key biomarker for prostate cancer detection.
• Label-free biosensing methods are crucial for efficient and sensitive biomarker detection.
• Surface Plasmon Resonance (SPR) biosensors offer high sensitivity for real-time molecular interactions.

Purpose of the Study:

• To develop and validate an amplitude-sensitive paired surface plasma wave biosensor (PSPWB) for label-free PSA detection.
• To assess the sensitivity, dynamic range, and performance of the PSPWB in buffer and human serum.
• To demonstrate the potential of PSPWB for clinical diagnostics.

Main Methods:

• Utilized an amplitude-sensitive paired surface plasma wave biosensor (PSPWB).
• Performed real-time detection of prostate-specific antigen (PSA).
• Analyzed PSA in phosphate buffered saline (PBS) and diluted human serum samples.

Main Results:

• Achieved a detection limit of 8.4 x 10(-9) refractive index unit (RIU).
• Successfully measured PSA from 10 fg/mL to 100 pg/mL with a linear SPR response.
• Demonstrated a wide dynamic range of 5 orders of magnitude for PSA concentration.
• Validated PSA detection in diluted human serum samples.

Conclusions:

• The PSPWB demonstrates high sensitivity and a broad dynamic range for PSA detection.
• Label-free, real-time detection of biological analytes in clinical samples is feasible with PSPWB.
• PSPWB offers a promising platform for simplified and efficient clinical diagnostic assays.