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Published on: February 15, 2020
Oxidative stress markers in patients with primary open-angle glaucoma
Asaad A Ghanem1, Lamiaa F Arafa, Ayman El-Baz
1Department of Ophthalmology, Faculty of Medicine, Mansoura University, Egypt. asaadghanem@hotmail.com
Current Eye Research
|April 9, 2010
Summary
Elevated levels of glutathione peroxidase (GPO), superoxide dismutase (SOD), and malondialdehyde (MDA) in aqueous humor are linked to primary open-angle glaucoma (POAG). MDA levels correlate with severe vision loss in POAG patients.
Area of Science:
- Ophthalmology
- Biochemistry
- Oxidative Stress Research
Background:
- Primary open-angle glaucoma (POAG) is a leading cause of irreversible blindness.
- Oxidative stress plays a role in the pathogenesis of glaucoma.
- Antioxidant enzyme status in the aqueous humor of POAG patients requires further investigation.
Purpose of the Study:
- To quantify antioxidant enzymes catalase (CAT), glutathione peroxidase (GPO), and superoxide dismutase (SOD), and malondialdehyde (MDA) in human aqueous humor.
- To determine if these markers differ between POAG patients and controls.
- To correlate enzyme levels with glaucoma severity.
Main Methods:
- Prospective case-control study design.
- Aqueous humor samples collected from 30 POAG patients and 25 age/gender-matched cataract patients.
- Analysis of CAT, GPO, SOD, and MDA levels in collected samples.
Main Results:
- Significantly elevated GPO, SOD, and MDA levels were observed in POAG patients compared to controls (P < 0.001).
- No significant difference in CAT enzyme activity was found between groups (P = 0.201).
- MDA levels showed a significant correlation with severe visual field loss in POAG patients (P < 0.001).
Conclusions:
- Increased aqueous humor GPO, SOD, and MDA may be associated with POAG.
- These elevated antioxidant markers might be a consequence of glaucoma rather than a primary cause.
- MDA levels could serve as a potential biomarker for glaucoma severity.
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