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Published on: March 31, 2021
Bioactivity-guided separation of anti-acidogenic substances against Streptococcus mutans UA 159 from Polygonum
1Department of Preventive Dentistry, School of Dentistry, Institute of Oral Bioscience and BK 21 Program, Chonbuk National University, Jeonju, Korea.
Objectives:
The aim of this study was to separate the anti-acidogenic substances against Streptococcus mutans UA 159 from Polygonum cuspidatum.
Material And Methods:
The anti-acidogenic substances were separated by a series of liquid-liquid fractionations followed by normal-phase silica gel liquid chromatography, based on high-performance liquid chromatography and glycolytic pH-drop assay. The effectiveness of the separated substances on the acidogenicity of Streptococcus mutans UA 159 was examined using sodium fluoride as a positive control. The chemical composition and quantities of the components of the substances was also assessed by qualitative-quantitative chromatographic analysis.
Results:
Among the substances separated from P. cuspidatum, F3 showed the strongest inhibitory effect on the acidogenicity of S. mutans UA 159 in a dose-dependent manner without displaying any bactericidal activity. F3 decreased the acidogenicity of S. mutans even at 12.5 microg ml(-1) (P < 0.05). F3 consisted mainly of resveratrol and emodin (C(14)H(12)O(3) and C(14)H(4)O(2)(OH)(3)CH(3), respectively), which made up approximately 60% of the weight of F3.
Conclusion:
F3 can be considered as a promising agent for controlling the acidogenicity of S. mutans and subsequent dental caries formation.
