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Sugars are under light control during bud burst in Rosa sp
Tiffanie Girault1, Farouk Abidi, Monique Sigogne
1Université d'Angers, UFR Sciences, UMR-462 SAGAH, 2, Bd Lavoisier, F-49045 Angers Cedex, France.
Plant, Cell & Environment
|April 9, 2010
Summary
Light is essential for rose bud burst, regulating sugar metabolism and gene activity. Darkness inhibits bud burst and triggers survival mechanisms, impacting glucidic pathways.
Area of Science:
- Plant Physiology
- Molecular Biology
- Biochemistry
Background:
- Bud burst is influenced by environmental cues, particularly light.
- The role of light in the physiological processes of bud burst is not fully understood.
- Roses exhibit an absolute requirement for light for bud burst.
Purpose of the Study:
- To investigate the impact of light on glucidic metabolism during bud burst in roses.
- To elucidate the molecular mechanisms underlying light-dependent bud burst.
Main Methods:
- Decapitated Rosa hybrida L. 'Radrazz' plants were exposed to darkness, white, blue, or red light.
- Measurements of sucrose reserves, vacuolar acid invertase activity, and gene transcription were performed.
- Analysis focused on the RhVI (vacuolar acid invertase) and RhNAD-SDH (NAD-dependent sorbitol dehydrogenase) genes.
Main Results:
- Sucrose mobilization and bud burst occurred only in the presence of light.
- Light strongly stimulated vacuolar acid invertase (RhVI) activity and RhVI gene transcription.
- Darkness led to reduced RhVI activity and transcription but strongly induced RhNAD-SDH gene transcription.
Conclusions:
- Light is crucial for initiating bud burst in roses by regulating sucrose metabolism.
- The RhVI gene is light-responsive and plays a key role in bud burst.
- The RhNAD-SDH gene's induction in darkness suggests a role in plant survival under light deprivation.
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