Pseudomonas spp. convert metmyoglobin into deoxymyoglobin
Related Experiment Video
Updated: Jun 14, 2026

Qualitative and Quantitative Analysis of Siderophore Production from Pseudomonas aeruginosa
Published on: March 15, 2024
Michiyo Motoyama1, Miho Kobayashi, Keisuke Sasaki
1Animal Products Research Team, National Institute of Livestock and Grassland Science, Tsukuba-shi, Ibaraki-ken, Japan. mmichiyo@affrc.go.jp
Abstract:
Meat 'reddening' by bacteria was observed in chilled beef. To identify the reddening bacteria, isolates were inoculated onto beef and the changes in CIE L*a*b* values monitored. As a result, two Pseudomonas spp., including Pseudomonas fragi which is commonly observed in raw meat, were selected and identified as reddening bacteria. The reddening was coincidentally occurred with the appearance of slime, and the increase in thiobarbituric acid-reactive substances (TBARS) was simultaneously suppressed. In myoglobin-containing nutrient broth, it is shown spectroscopically that P. fragi converted metmyoglobin into deoxymyoglobin. It was concluded that the meat reddening was due to the formation of deoxymyoglobin, induced by the very-low-oxygen tension brought about by Pseudomonad's oxygen consumption: This oxygen depletion simultaneously suppressed TBARS increase.
More Related Videos
11:00Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress
Published on: August 31, 2015
11:32Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria
Published on: February 23, 2014
Related Concept Videos
Metabolism of Chemolithotrophs
Anoxygenic Photosynthesis
Pyruvate Oxidation
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
Anoxygenic Phototrophic Bacteria
Microbes and Other Elemental Cycles
Microbial Corrosion