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Updated: Jun 14, 2026
13:33
Infinium Assay for Large-scale SNP Genotyping Applications
Published on: November 19, 2013
Development of breed identification markers based on a bovine 50K SNP array
1Laboratory of Animal Breeding and Genetics, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan.
Meat Science
|April 9, 2010
Summary
New genetic markers can distinguish Japanese from US cattle breeds, helping prevent mislabeled beef sales. This food safety advancement utilizes a bovine 50K SNP array for accurate breed identification.
Area of Science:
- Animal genetics
- Food safety
- Agricultural science
Background:
- Japanese Black and Holstein cattle are key domestic meat sources in Japan.
- Imported beef from Australia and the US is also in demand.
- The Bovine Spongiform Encephalopathy (BSE) outbreak highlighted issues of mislabeled imported beef as domestic, impacting consumer trust and food safety.
Purpose of the Study:
- To develop genetic markers for discriminating between Japanese and US cattle breeds.
- To enhance food safety by preventing the false sale of imported beef.
Main Methods:
- Utilized a bovine 50K SNP (Single Nucleotide Polymorphism) array.
- Developed and validated five US-specific SNP markers (BISNP7, BISNP15, BISNP21, BISNP23, and BISNP26).
Main Results:
- Five US-specific markers were identified with allelic frequencies ranging from 0.102 to 0.250.
- The combined use of these five markers achieved a 0.858 probability of correctly identifying US cattle.
- The bovine 50K SNP array proved effective for developing breed identification markers.
Conclusions:
- The developed SNP markers are effective tools for distinguishing Japanese and US cattle breeds.
- These markers can significantly contribute to preventing falsified beef displays in Japan.
- The study demonstrates the utility of SNP arrays in ensuring beef authenticity and consumer safety.
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