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Updated: Jun 14, 2026

Reconstitution of Msp1 Extraction Activity with Fully Purified Components
Published on: August 10, 2021
Asna1/TRC40-mediated membrane insertion of tail-anchored proteins
Vincenzo Favaloro1, Fabio Vilardi, Rainer Schlecht
1Zentrum für Molekulare Biologie der Universität Heidelberg, DKFZ - ZMBH Allianz, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany.
Abstract:
Tail-anchored (TA) proteins insert post-translationally into the membrane of the endoplasmic reticulum (ER) and span the membrane by their C-terminal transmembrane domain. We have reconstituted membrane insertion of TA proteins from recombinant Asna1/TA protein complexes and ER-derived membranes. Our data show that Asna1 can mediate membrane insertion of RAMP4 and Sec61beta without the participation of other cytosolic proteins by a mechanism that depends on the presence of ATP or ADP and a protease-sensitive receptor in the ER membrane. By contrast, membrane insertion of cytochrome b5 can proceed independently of Asna1 and nucleotides.
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