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Updated: Jun 14, 2026

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis
Published on: June 25, 2020
Two closely related subclass II SnRK2 protein kinases cooperatively regulate drought-inducible gene expression
Masahide Mizoguchi1, Taishi Umezawa, Kazuo Nakashima
1RIKEN Plant Science Center, 3-1-1, Kouyadai, Tsukuba, Ibaraki, 305-0074 Japan.
Abstract:
The subclass III group of SNF1-related protein kinase 2 (SnRK2) members is known to play an important role in ABA and osmotic stress signaling in Arabidopsis; however, the roles of other subclasses remain elusive. Here, we established a double mutant of SRK2C/SnR2.8 and SRK2F/SnRK2.7 to investigate the functions of subclass II SnRK2s. Microarray analysis suggested that subclass II SnRK2s regulate some drought-responsive genes involving ABA-responsive element binding transcription factors (AREB/ABF) and their targets, and quantitative reverse transcription- PCR confirmed that those genes were down-regulated significantly in srk2cf. This study indicates that subclass II SnRK2s also play important roles in drought stress signaling in Arabidopsis.
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