Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple mRNAs encode the murine translation initiation factor eIF-4E.

M Jaramillo1, J Pelletier, I Edery

  • 1Department of Biochemistry, McGill University, Montreal, Federal Republic of Germany.

The Journal of Biological Chemistry
|June 5, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

"Time is Brain" also in multiple sclerosis: Fifteen years later, a fully consolidated concept.

Revue neurologique·2026
Same author

Immune checkpoint inhibitor - associated optic neuropathy: Two illustrative cases and therapeutic implications.

Revue neurologique·2026
Same author

Long-interval dosing of anti-CD20 therapy: Toward affordable treatment for multiple sclerosis.

Revue neurologique·2026
Same author

Osteopathic manipulation and its applicability in the emergency department: A narrative review.

The American journal of emergency medicine·2024
Same author

Contribution of the MP2RAGE 7T Sequence in MS Lesions of the Cervical Spinal Cord.

AJNR. American journal of neuroradiology·2023
Same author

Impact of methodological choices in comparative effectiveness studies: application in natalizumab versus fingolimod comparison among patients with multiple sclerosis.

BMC medical research methodology·2022
Same journal

Wanted and unwanted modifications of mRNA, and their effect on gene expression and signaling.

The Journal of biological chemistry·2026
Same journal

TGF-β2 drives lipid droplet accumulation in chondrocytes through the TβRI/p-Smad3/Fabp5 axis.

The Journal of biological chemistry·2026
Same journal

Macrophage-specific targeting of histone demethylases with small-molecule inhibitors suppresses inflammatory response in vivo.

The Journal of biological chemistry·2026
Same journal

Substrate and target selectivity of 4'-fluoroadenosine against viral and host polymerases.

The Journal of biological chemistry·2026
Same journal

Correction: Characterization of Mast2 kinase defines structural features, regulation, and substrates.

The Journal of biological chemistry·2026
Same journal

Isotope-Edited ESEEM: A New Method for Probing Copper Binding Sites in Neurodegenerative Proteins.

The Journal of biological chemistry·2026
See all related articles

Eukaryotic messenger RNAs have a 5' cap structure essential for translation. This study reveals multiple messenger RNA forms for eukaryotic initiation factor 4E (eIF-4E), impacting cellular growth and differentiation.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • The 5' cap structure (m7GpppX) on eukaryotic mRNAs is crucial for translation initiation.
  • Initiation factor-4F (eIF-4F), specifically the eIF-4E subunit, binds the cap and facilitates mRNA secondary structure melting.
  • eIF-4E levels are hypothesized to regulate translation initiation rates, influencing cell growth and differentiation.

Purpose of the Study:

  • To investigate the regulation of eukaryotic initiation factor 4E (eIF-4E) expression.
  • To determine if eIF-4E expression patterns vary across different cell types and tissues.

Main Methods:

  • Analysis of eIF-4E mRNA expression patterns in various cell lines.
  • Investigation of differential polyadenylation as a mechanism for generating multiple eIF-4E mRNA variants.

Related Experiment Videos

  • Examination of tissue-specific differences in eIF-4E mRNA expression and polyadenylation site usage.

    • Main Results:

    • Demonstrated the existence of multiple eIF-4E messenger RNA (mRNA) transcripts.
    • Showed that these multiple mRNAs are generated through differential polyadenylation.
    • Identified tissue-specific variations in eIF-4E mRNA expression and the utilization of distinct polyadenylation sites.

    Conclusions:

    • eIF-4E expression is regulated at the post-transcriptional level through differential polyadenylation.
    • Tissue-specific expression patterns suggest a role for eIF-4E in specialized cellular functions.
    • Understanding eIF-4E mRNA regulation is key to comprehending control of cell growth and differentiation.