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Related Experiment Video

Updated: Jun 14, 2026

Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo
07:46

Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo

Published on: October 11, 2022

Microdevice to capture colon crypts for in vitro studies.

Yuli Wang1, Rahul Dhopeshwarkar, Rani Najdi

  • 1Department of Chemistry, University of North Carolina, Chapel Hill, NC 27599, USA.

Lab on a Chip
|April 9, 2010
PubMed
Summary
This summary is machine-generated.

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Researchers developed a microengineered device for capturing and orienting colon crypts, enabling controlled fluidic microenvironments for biological assays. This technology supports studying tissue in conditions mimicking the in vivo state.

Area of Science:

  • BioMEMs (Biological and Microelectromechanical Systems)
  • Tissue Engineering
  • Microfluidics

Background:

  • Biological research requires tools to manipulate microscale tissue environments.
  • Colon crypts are important but under-studied tissues.
  • Existing methods lack precise control over the microenvironment of isolated tissues.

Purpose of the Study:

  • To design and test a BioMEMs device for oriented capture of colon crypts.
  • To enable biological assays of living colonic crypts in user-controlled fluidic microenvironments.
  • To recapitulate the in vivo state for studying polarized tissue.

Main Methods:

  • Development of a device with micron-scale capture sites.
  • Testing with polymeric crypt surrogates for initial design validation.

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Last Updated: Jun 14, 2026

Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo
07:46

Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo

Published on: October 11, 2022

Cryosectioning Method for Microdissection of Murine Colonic Mucosa
06:16

Cryosectioning Method for Microdissection of Murine Colonic Mucosa

Published on: July 12, 2015

Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures
08:53

Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures

Published on: November 3, 2014

  • Evaluation using isolated colon crypts from murine models.
  • Utilizing inert glass beads to block unfilled capture sites.
  • Demonstration of chemical concentration gradients across arrayed crypts.

    • Main Results:

    • High capture efficiency (92+/-3%) and orientation of crypt surrogates.
    • Successful capture of fixed colon crypts (37+/-5%) with high orientation (94+/-3%).
    • Establishment of a stable chemical concentration gradient (Cluminal/Cbasal>10) for over 8 hours.
    • Demonstrated viability of unfixed colon crypts on the device for 5 hours post-sacrifice.

    Conclusions:

    • The microengineered device enables efficient, oriented capture of colon crypts.
    • The technology facilitates the creation of controlled microenvironments for colon crypt research.
    • This approach shows feasibility for advanced biological assays and tissue studies.