Related Experiment Video
Updated: Jun 14, 2026
Fabrication of the Composite Regenerative Peripheral Nerve Interface (C-RPNI) in the Adult Rat
Published on: February 25, 2020
Oxidized regenerated cellulose does not prevent the formation of experimental postoperative perineural fibrosis
Pedro Hernández-Cortés1, Magdalena Peregrina, Jose Aneiros-Fernández
1Department of Traumatology and Orthopaedic Surgery, IBIMER, "San Cecilio" Clinical Hospital and University of Granada, Spain. hdezcp@hotmail.com
Introduction:
It is difficult to prevent and treat intra- and peri-neural fibrosis after peripheral nerve surgery. Many authors have attempted to develop and verify the effectiveness of substances to decrease the formation of adherences in different tissues.
Material And Methods:
this study aimed to assess the effectiveness of a barrier of oxidized regenerated cellulose (ORC) to reduce adherence and perineural fibrosis in a model of surgical perineural induced fibrosis in rat sciatic nerve in 40 rats. After tissue aggression, the nerve of the right rear limb was wrapped in ORC and the left limb served as control. Animals were killed at 3 and 6 weeks, and nerves and muscle mass were extracted en bloc. Connective tissue was quantified by conventional histopathological techniques and Fibrosis HR(R) automatic image analysis.
Results:
No significant differences were found in intra- or peri-neural induced fibrosis between control nerves (6.88% and 8.90%, respectively) and treated nerves (6.57% and 9.90%) at 3 or 6 weeks (10.41% and 12.51% in controls; 11.85% and 15.72% in treated nerves). Inflammatory phenomena and granulomatous reactions were more frequent in treated animals.
Conclusions:
ORC conferred no advantage in prevention of nerve fibrosis and might have interfered with healing.
