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Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)

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Published on: June 14, 2012

[Zinc finger nucleases and their application].

Shan-shan Deng¹, Ying-zhi Wang, Duan Ma

¹Key Laboratory of Molecular Medicine, Ministry of Education, Shanghai Medical College, Fudan University, Shanghai, P.R. China.

Zhonghua Yi Xue Yi Chuan Xue Za Zhi = Zhonghua Yixue Yichuanxue Zazhi = Chinese Journal of Medical Genetics

|April 9, 2010

Summary

Zinc finger nucleases (ZFNs) are engineered proteins that create targeted DNA breaks, enabling genetic modifications through cellular repair pathways. This review covers ZFN methodology and their advantages in genetic research.

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Mouse Genome Engineering Using Designer Nucleases

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Published on: April 2, 2014

Area of Science:

Molecular Biology
Gene Editing Technologies
Biotechnology

Context:

Zinc finger nuclease (ZFN) technology utilizes engineered DNA-binding proteins fused to a nuclease domain.
ZFNs create targeted double-stranded DNA breaks (DSBs) at specific genomic loci.
These DSBs are repaired by cellular mechanisms like homologous recombination (HR) or nonhomologous end joining (NHEJ), leading to desired mutations.

Purpose:

To review the methodology and technical advantages of Zinc finger nucleases (ZFNs).
To highlight the application of ZFNs in genetic research.
To provide an overview of ZFN-based gene editing strategies.

Summary:

Zinc finger nucleases (ZFNs) are chimeric proteins combining Cys2-His2 zinc-finger proteins with the Fok I endonuclease cleavage domain.
ZFNs induce targeted double-stranded breaks (DSBs) in DNA.
Subsequent repair via HR or NHEJ results in genetic mutations, making ZFNs a powerful tool in genetic research.

Impact:

ZFNs offer a versatile platform for targeted genome modification.
Understanding ZFN methodology is crucial for advancing genetic engineering and research applications.
The review emphasizes the utility and benefits of ZFNs in modern biological studies.