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Related Experiment Video

Updated: Jun 14, 2026

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

Published on: October 30, 2019

Collagen-induced arthritis.

Edward F Rosloniec¹, Michael Cremer¹, Andrew H Kang¹

¹Veterans Affairs Medical Center, and University of Tennessee Health Science Center, Memphis, Tennessee.

Current Protocols in Immunology

|April 9, 2010

Summary

This summary is machine-generated.

This study details the collagen-induced arthritis (CIA) mouse model, a key tool for rheumatoid arthritis research. It provides protocols for reproducible induction and evaluation of CIA in mice.

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A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

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Published on: October 30, 2019

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Area of Science:

Immunology
Rheumatology
Animal Models

Background:

Rheumatoid arthritis (RA) is a chronic autoimmune disease.
The collagen-induced arthritis (CIA) mouse model is a standard preclinical tool for studying RA pathogenesis and testing therapeutics.
Reproducible induction and accurate assessment of CIA are crucial for reliable research outcomes.

Purpose of the Study:

To provide a comprehensive guide for researchers on establishing and evaluating the collagen-induced arthritis (CIA) mouse model.
To detail the necessary steps for reproducible induction of CIA in genetically susceptible mouse strains.
To outline methods for assessing the incidence and severity of arthritis in this model.

Main Methods:

Acquisition, handling, and preparation of type II collagen (CII).
Selection of appropriate mouse strains susceptible to CIA.
Standardized immunization techniques using CII emulsified in complete Freund's adjuvant.
Evaluation methods for arthritis incidence and severity.

Main Results:

The CIA model allows for the induction of autoimmune arthritis in mice.
Arthritis onset typically occurs 21–28 days post-immunization.
The described protocols facilitate reproducible CIA induction and evaluation.

Conclusions:

This unit offers essential information for investigators to reliably induce and assess CIA in mice.
The CIA model serves as a valuable platform for understanding RA and developing new treatments.
Standardized protocols ensure consistency and comparability in CIA research.