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Positron Emission Tomography Using 64-Copper as a Tracer for the Study of Copper-Related Disorders
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Positron Emission Tomography Using 64-Copper as a Tracer for the Study of Copper-Related Disorders

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Development of highly sensitive fluorescent probes for detection of intracellular copper(I) in living systems

Masayasu Taki1, Shohei Iyoshi, Akio Ojida

  • 1Graduate School of Human and Environmental Studies, Kyoto University, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan. taki@chem.mbox.media.kyoto-u.ac.jp

Journal of the American Chemical Society
|April 10, 2010
PubMed

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...