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Updated: Jun 14, 2026
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Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue
Published on: September 2, 2013
Histo-proteomic profiling of formalin-fixed, paraffin-embedded tissue
Kant M Matsuda1, Joon-Yong Chung, Stephen M Hewitt
1Tissue Array Research Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-4605, USA.
Expert Review of Proteomics
|April 10, 2010
Summary
Advancements in proteomic profiling of clinical tissues enable biomarker discovery. New methods integrate tissue structure information, improving the analysis of formalin-fixed samples for disease prediction.
Area of Science:
- Proteomics and Bioinformatics
- Clinical Pathology
- Biomarker Discovery
Background:
- Proteomic profiling of annotated tissues is crucial for identifying disease biomarkers.
- Developing predictive biomarkers, especially for cancer, necessitates direct tissue analysis.
- Previous proteomic methods yielded unsatisfactory results on formalin-fixed, paraffin-embedded tissues.
Purpose of the Study:
- To discuss recent technological advancements in proteomic profiling for clinical applications.
- To highlight approaches that retain histomorphologic information during proteomic analysis.
Main Methods:
- Review of recent advancements in proteomic profiling technologies.
- Focus on methods compatible with clinical tissue specimens (formalin-fixed, paraffin-embedded).
- Integration of histomorphologic information into proteomic analysis workflows.
Main Results:
- Emerging proteomic technologies show promise for analyzing clinical tissue samples.
- Methods preserving tissue morphology enhance the utility of proteomic data.
- Improved analytical techniques address limitations of previous proteomic studies on fixed tissues.
Conclusions:
- Recent advancements offer improved proteomic profiling of clinical tissues.
- Integrating histomorphologic information is key to successful biomarker discovery in fixed tissues.
- These advancements facilitate the translation of research biomarkers to clinical practice.
