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The density of clonogenic cells in human solid tumors
International Journal of Cell Cloning
|March 1, 1991
Summary
Tumor clonogenic cell density is crucial for cancer therapy response. An enriched monolayer assay confirms approximately 1.12 x 10^5 clonogens per gram in human tumors, aligning with indirect radiation response methods.
Area of Science:
- Oncology
- Cancer Biology
- Cell Biology
Background:
- Tumors are sustained by clonogenic cells, which are the primary targets in cancer therapy.
- Quantifying clonogenic cell density in tumors is essential for evaluating therapeutic response.
- Previous methods for determining clonogen density yielded inconsistent results.
Purpose of the Study:
- To accurately determine the density of clonogenic cells in human tumors.
- To validate a new enriched monolayer clonogenic assay for this purpose.
Main Methods:
- Enzymatic disaggregation of human tumor biopsies.
- Assaying enzyme digests for clonogenic cells using an enriched monolayer clonogenic assay.
- Comparing results with indirect methods based on tumor radiation response.
Main Results:
- The enriched monolayer clonogenic assay determined an average clonogen density of 1.12 x 10^5 clonogens per gram of tumor tissue.
- This density exhibited a wide range across different tumor samples.
- The results closely agreed with previously reported indirect methods.
Conclusions:
- The enriched monolayer clonogenic assay reliably quantifies clonogenic cell density in human tumors.
- This assay supports the proliferation of tumor-maintaining cells, validating its use in cancer research.
- Accurate clonogen density measurement can significantly impact cancer therapy assessment.