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Updated: Jun 14, 2026
11:36
Glycan Node Analysis: A Bottom-up Approach to Glycomics
Published on: May 22, 2016
Common aberrations from the normal human plasma N-glycan profile.
Maja Pucic1, Sofia Pinto, Mislav Novokmet
1Genos Ltd., Glycobiology Division, Planinska 1, 10000 Zagreb, Croatia.
Glycobiology
|April 10, 2010
Summary
Researchers identified distinct N-glycan profiles in plasma, revealing specific glyco-phenotypes. Some altered patterns, like A2G2S1, are linked to health issues, while others show no adverse effects.
Area of Science:
- Biochemistry
- Human Genetics
- Clinical Chemistry
Background:
- N-glycans are crucial glycans involved in various biological processes.
- Altered N-glycan profiles in plasma can indicate underlying health conditions.
Purpose of the Study:
- To identify and characterize distinct N-glycan profiles in human plasma.
- To investigate the association between specific N-glycan patterns and clinical conditions.
Main Methods:
- Hydrophilic interaction and weak anion exchange high-performance liquid chromatography (HILIC-WAX-HPLC) were used to analyze plasma N-glycans.
- Consensus scoring of pairwise distances was applied to group individuals based on glycan profiles.
Main Results:
- Six distinct glyco-phenotype groups were identified among 1991 individuals.
- An increase in monosialylated biantennary glycans (A2G2S1) was associated with renal problems.
- Significant alterations, including the absence of neutral glycans or fucosylated glycans, were not linked to adverse health outcomes.
Conclusions:
- Specific altered glyco-phenotypes exist in the human population.
- Certain glyco-phenotypes may serve as risk factors for developing specific diseases.
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