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Updated: Jun 14, 2026

From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins
Published on: July 4, 2016
Bacterial Adaptor Membrane Fusion Proteins and the Structurally Dissimilar Outer Membrane Auxiliary Proteins Have
Anthony Y Xiao1, Jing Wang, Milton H Saier
1Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093-0116, USA.
Abstract:
Transport systems frequently include auxiliary proteins that perform subfunctions within the transporter protein complex. Two such proteins found in Gram-negative bacteria are the Membrane Fusion Proteins (MFPs) and the Outer Membrane Auxiliary (OMA) proteins. We here demonstrate that OMAs present in alpha-proteobacteria (but not in other bacterial types) contain a long alpha-helical region that is homologous to corresponding regions in the MFPs. The results suggest that during their evolution, OMAs, specifically from alpha-proteobacteria, exchanged their own alpha-helical domain for one derived from an MFP. The structural and functional implications of these findings are discussed.
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