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Updated: Jun 14, 2026
Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles
Published on: October 13, 2017
Expression of HSP70 and Its Relation with Other Cytokines in Human Middle Ear Effusion
Shi-Nae Park1, Kyoung Ho Park, Hyeog-Gi Choi
1Department of Otorhinolaryngology-Head and Neck Surgery, The Catholic University of Korea School of Medicine, Seoul, Korea.
Objectives:
While other cytokines are known to be associated with otitis media with effusion (OME), the involvement of heat shock protein 70 (HSP70) in middle ear effusion (MEE) is unknown. This study was undertaken to investigate the possibility of there being a HSP70 expression in human MEE and to determine its potential role as a cytokine in OME.
Methods:
The levels of HSP70, tumor necrosis factor-alpha and interleukin-1beta were measured by enzyme-linked immunosorbent assay in the effusion of different groups of OME patient following collection of the MEE using our new collection system. The clinical characteristics of the OME patients and the MEE status were analyzed.
Results:
HSP70 was expressed in all the types of MEE. The mucous and seromucous effusions showed higher HSP70 levels than that of the serous effusion. The HSP70 level was correlated with the levels of tumor necrosis factor (TNF)-alpha and interleukin (IL)-1beta in the effusions. The positive correlations between HSP70, TNF-alpha and IL-1beta were statistically significant (P<0.05).
Conclusion:
The highly elevated level of HSP70 in the seromucous and mucous effusions implicates this protein in the chronicity of OME.
Insights
Heat shock protein 70 (HSP70) is present in middle ear effusion (MEE) and correlates with other cytokines. Elevated HSP70 levels in mucous and seromucous MEE suggest its role in chronic otitis media with effusion (OME).
Area of Science:
- Otolaryngology
- Immunology
- Molecular Biology
Background:
- Otitis media with effusion (OME) is associated with various cytokines.
- The role of heat shock protein 70 (HSP70) in middle ear effusion (MEE) remains uninvestigated.
Purpose of the Study:
- To determine HSP70 expression in human MEE.
- To explore HSP70's potential role as a cytokine in OME.
Main Methods:
- Collected MEE from OME patients using a novel system.
- Quantified HSP70, TNF-alpha, and IL-1beta levels via ELISA.
- Analyzed clinical data and MEE characteristics.
Main Results:
- HSP70 was detected in all MEE types.
- Mucous and seromucous effusions exhibited higher HSP70 levels than serous effusions.
- Significant positive correlations were found between HSP70, TNF-alpha, and IL-1beta levels (P<0.05).
Conclusions:
- HSP70 is expressed in human MEE.
- Elevated HSP70 in specific MEE types suggests involvement in OME chronicity.
