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Related Concept Videos

Selectins01:25

Selectins

Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain, which...

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Related Experiment Video

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A Flow Adhesion Assay to Study Leucocyte Recruitment to Human Hepatic Sinusoidal Endothelium Under Conditions of Shear Stress
11:38

A Flow Adhesion Assay to Study Leucocyte Recruitment to Human Hepatic Sinusoidal Endothelium Under Conditions of Shear Stress

Published on: March 21, 2014

Leucocyte adhesion under haemodynamic flow conditions.

Charlotte Lawson1, Marlene Rose, Sabine Wolf

  • 1Veterinary Basic Sciences, Royal Veterinary College, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|April 10, 2010
PubMed
Summary

This study introduces a novel in vitro model to investigate how prolonged exposure to blood flow affects endothelial cells (EC) and leukocytes. The system allows for studying the dynamic interactions crucial for understanding vascular health and disease.

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Last Updated: Jun 14, 2026

A Flow Adhesion Assay to Study Leucocyte Recruitment to Human Hepatic Sinusoidal Endothelium Under Conditions of Shear Stress
11:38

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Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay
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Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay

Published on: December 30, 2014

Area of Science:

  • Vascular biology
  • Cellular biomechanics
  • Immunology

Background:

  • Endothelial cells (EC) form a barrier lining blood vessels, constantly sensing blood flow and tissue signals.
  • Current understanding of EC function often relies on static culture, neglecting the impact of dynamic fluid shear stress.
  • Fluid shear stress, generated by blood friction against EC, is a critical factor in vascular health.

Purpose of the Study:

  • To present a methodology for an in vitro system to study shear stress effects on EC.
  • To investigate the impact of prolonged leukocyte co-culture under flow conditions.
  • To enable research on endothelial and leukocyte populations under dynamic physiological stimuli.

Main Methods:

  • Development of an in vitro system for recirculating cell culture medium and leukocytes over endothelium.
  • Application of controlled fluid shear stress mimicking physiological conditions.
  • Long-term co-culture experiments ranging from minutes to days.

Main Results:

  • The described system allows for the study of endothelial cell responses to prolonged shear stress.
  • It facilitates the investigation of leukocyte-endothelial cell interactions under flow.
  • Provides a platform to analyze changes in both endothelial and leukocyte populations over time.

Conclusions:

  • The developed in vitro model overcomes limitations of static cultures for studying EC function.
  • It offers a valuable tool for exploring the complex interplay between blood flow, EC, and leukocytes.
  • This methodology aids in understanding vascular dysfunction and inflammatory processes.