Isolation of outer membrane proteins (OMP) from Salmonella cells using zwitterionic detergent and their separation by
Bozena Futoma-Kołoch1, Gabriela Bugla-Płoskońska, Włodzimierz Doroszkiewicz
1Department of Microbiology, Institute of Genetics and Microbiology, University of Wroclaw, Poland. bozena.futoma-koloch@microb.uni.wroc.pl
Abstract:
We report that using the zwitterionic detergent Zwittergent Z 3-14 to isolate outer membrane proteins (OMPs) from Salmonella 048 is suitable for their separation by two-dimensional electrophoresis (2-DE) in a capillary tube system. Sample preparation is a very crucial step for any bacterial proteomic study. Some modifications were introduced to the 2-DE protocol suggested by O'Farrell and BioRad, which significantly impaired the resolution of proteins. 2-DE analysis of OMPs may be helpful in the interpretation of the variable susceptibility of Salmonella 048 rods to the bactericidal activity of serum.
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