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Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their access...

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Updated: Jun 14, 2026

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)

Published on: December 24, 2015

Human mesenchymal stem cells efficiently manage oxidative stress.

Araceli Valle-Prieto¹, Paulette A Conget

¹Instituto de Ciencias, Facultad de Medicina Clinica Alemana, Universidad del Desarrollo, Santiago, Chile.

Stem Cells and Development

|April 13, 2010

Summary

Mesenchymal stem cells (MSCs) effectively manage oxidative stress (OS) by scavenging reactive oxygen and nitrogen species. This inherent capability suggests their potential for in vivo tissue regeneration and reducing OS-related damage.

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Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)

Published on: December 24, 2015

Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer

Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer

Published on: September 24, 2021

Area of Science:

Stem cell biology
Oxidative stress research
Regenerative medicine

Background:

Mesenchymal stem cells (MSCs) show therapeutic promise for conditions involving tissue damage and oxidative stress (OS).
Understanding the intrinsic mechanisms by which MSCs handle OS is crucial for their clinical application.

Purpose of the Study:

To investigate the in vitro capacity of primary human MSCs (hMSCs) to manage oxidative stress.
To assess hMSC viability, reactive species levels, antioxidant enzyme activity, and glutathione status under OS conditions.

Main Methods:

In vitro assessment of hMSC viability upon exposure to reactive oxygen species (ROS) and reactive nitrogen species (RNS).
Measurement of intracellular reactive species, basal gene expression and activity of antioxidant enzymes (superoxide dismutases, catalase, glutathione peroxidase), and total glutathione (GSx) levels.
Evaluation of GSx-depleted hMSC viability under ROS/RNS exposure.

Main Results:

hMSCs exhibit high resistance to OS-induced cell death.
This resistance is linked to low intracellular reactive species, constitutive antioxidant enzyme expression, and high GSx levels.
GSx depletion abrogated the OS management capacity of hMSCs.

Conclusions:

In vitro, hMSCs effectively scavenge ROS and RNS, demonstrating robust OS management capabilities.
The findings suggest that hMSCs could contribute to in vivo tissue regeneration by mitigating OS-induced damage, provided this potential is maintained.