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Updated: Jun 14, 2026

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles

Published on: October 19, 2015

Engineered mesenchymal stem cells with self-assembled vesicles for systemic cell targeting.

Debanjan Sarkar¹, Praveen K Vemula, Weian Zhao

¹Harvard-MIT Division of Health Sciences and Technology, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Harvard Stem Cell Institute, 65 Landsdowne Street, Cambridge, MA 02139, USA.

|April 13, 2010

Summary

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Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their access...

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Researchers developed a new method using lipid vesicles to temporarily modify cell surfaces with adhesion ligands, improving cell rolling for better homing. This technique enhances cell targeting for therapies without affecting cell viability or differentiation.

Area of Science:

Biotechnology
Cell Biology
Regenerative Medicine

Background:

Cell therapy offers significant potential for patient treatment.
Systemic cell delivery via infusion faces engraftment efficiency challenges.
Current cell surface modification methods have safety and complexity limitations.

Purpose of the Study:

To develop a facile and generic approach for transient cell surface engineering.
To improve cell homing efficiency by promoting cell rolling.
To evaluate the use of lipid vesicles for presenting biomolecular ligands.

Main Methods:

Utilized lipid vesicles to transiently engineer cell surfaces.
Introduced biotin moieties for subsequent ligand conjugation (e.g., streptavidin).

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Last Updated: Jun 14, 2026

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles

Published on: October 19, 2015

Immobilized sialyl Lewis X (SLeX) on mesenchymal stem cells (MSCs) to study rolling on P-selectin under flow.

Main Results:

Lipid vesicles rapidly fuse with cell membranes, introducing functional moieties.
SLeX-modified MSCs exhibited significantly enhanced rolling (8 µm/s) compared to unmodified MSCs (61 µm/s) under shear stress.
Cell surface modification did not adversely affect MSC viability or differentiation potential.

Conclusions:

Transient cell surface modification with lipid vesicles is an effective strategy for ligand immobilization.
This approach can potentially improve the targeting of systemically administered cells to inflammatory sites.
The method offers a versatile platform for enhancing cell homing in various cell types.