Related Experiment Video
Updated: Jun 14, 2026

A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants
Published on: January 1, 2016
On-line solid phase extraction fast liquid chromatography-tandem mass spectrometry for the analysis of bisphenol A
H Gallart-Ayala1, E Moyano, M T Galceran
1Department of Analytical Chemistry, University of Barcelona, Martí i Franquès 1-11, Diagonal 647, 08028 Barcelona, Spain.
Abstract:
In this study an on-line column-switching fast LC-MS/MS method was developed to analyze bisphenol A (BPA) and its chlorinated derivatives in water. Fast liquid chromatographic separation was performed on a C18 reversed phase column based on fused-core particle technology (2.7 microm particle size) providing analysis times shorter than 3 min and high peak efficiencies. The main benefit of this LC system is that it can easily be hyphenated to a conventional on-line preconcentration device allowing the direct analysis of water samples without any pretreatment at concentrations levels down to 60 ng L(-1) and preventing contaminations frequently reported in the analysis of BPA. This on-line SPE fast LC system was coupled to a triple quadrupole mass spectrometer operating in enhanced mass resolution mode (Q1 FWHM=0.7 Th, Q3 FWHM=0.1 Th) in order to minimize interferences and chemical noise. This highly sensitive and selective method was successfully employed to analyze BPA and its chlorinated derivatives in water samples.
More Related Videos
04:39A Modified QuEChERS-HPLC Method for Detection of Polycyclic Aromatic Hydrocarbons in Zebrafish Embryos Exposed to Fine Particulate Matter
Published on: June 13, 2025
09:04Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow
Published on: April 18, 2019
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Types of Detectors
High-Performance Liquid Chromatography: Instrumentation
Analyte Adsorption and Distribution
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Gas Chromatography–Mass Spectrometry (GC–MS)
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall. The coating...