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Updated: Jun 14, 2026

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
14:43

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

Molecular recognition of DNA-protein complexes: a straightforward method combining scanning force and fluorescence

Humberto Sanchez1, Roland Kanaar, Claire Wyman

  • 1Department of Cell Biology and Genetics, Cancer Genomics Center, Erasmus MC, PO Box 2040, 3000 CA Rotterdam, The Netherlands.

Ultramicroscopy
|April 13, 2010
PubMed

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

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Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...