Optimization of data collection taking radiation damage into account
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Updated: Jun 14, 2026

An Automated Microscopic Scoring Method for the γ-H2AX Foci Assay in Human Peripheral Blood Lymphocytes
Published on: December 25, 2021
Gleb P Bourenkov1, Alexander N Popov
1EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85b, 22607 Hamburg, Germany.
Abstract:
To take into account the effects of radiation damage, new algorithms for the optimization of data-collection strategies have been implemented in the software package BEST. The intensity variation related to radiation damage is approximated by log-linear functions of resolution and cumulative X-ray dose. Based on an accurate prediction of the basic characteristics of data yet to be collected, BEST establishes objective relationships between the accessible data completeness, resolution and signal-to-noise statistics that can be achieved in an experiment and designs an optimal plan for data collection.
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