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Application of bipolar electrodialysis to E. coli fermentation for simultaneous acetate removal and pH control
Michael Wong1, John M Woodley, Gary J Lye
1Department of Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, UK.
Abstract:
The application of bipolar electrodialysis (BPED) for the simultaneous removal of inhibitory acetate and pH control during E. coli fermentation was investigated. A two cell pair electrodialysis module, consisting of cation exchange, anion exchange and bipolar membranes with working area of 100 cm(2) each, was integrated with a standard 7 l stirred tank bioreactor. Results showed that BPED was beneficial in terms of in situ removal of inhibitory acetate and a reduction in the amount NH(4)OH used for pH control. In batch and fed-batch BPED fermentations, base additions were decreased by up to 50% in both cases compared to electrodialysis (ED) fermentations with pH controlled at 6.7 +/- 0.1. Consequently, the final biomass (34.2 g DCW l(-1)) and recombinant protein (5.5 g l(-1)) concentrations obtained were increased by up to 37 and 20%, respectively.
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