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Related Experiment Video

Updated: Jun 13, 2026

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

[An enzyme sensor for phenolic compounds analysis].

Ye Li¹, Hui Yang, Wei-Wei Han

¹Department of Chemistry, School of Applied Science, University of Science and Technology Beijing, Beijing 100083, China. liye@sas.ustb.edu.cn

Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu

|April 14, 2010

Summary

This study immobilizes horseradish peroxidase (HRP) in a sol-gel matrix for phenolic compound analysis. The biosensor offers a recyclable method for spectrophotometric detection of phenols.

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Published on: April 22, 2022

Area of Science:

Biochemistry
Analytical Chemistry
Materials Science

Background:

Phenolic compounds are widespread environmental pollutants.
Accurate detection of phenols is crucial for environmental monitoring.
Horseradish peroxidase (HRP) is a key enzyme for catalyzing phenol oxidation.

Purpose of the Study:

To develop an immobilized enzyme biosensor for phenolic compound analysis.
To evaluate the stability and reusability of immobilized HRP.
To establish a spectrophotometric method for phenol detection.

Main Methods:

Immobilization of HRP in a sol-gel silica matrix.
Spectrophotometric detection of colored products formed by oxidative coupling.
BET analysis to characterize the porous structure of the silica matrix.

Main Results:

Immobilized HRP retained high enzymatic activity.
The sol-gel matrix allowed diffusion of reactants but retained the enzyme.
The biosensor demonstrated feasibility for analyzing chlorophenols.
Enzyme activity decreased after 9 reuse cycles, but the method showed potential.

Conclusions:

Sol-gel immobilized HRP provides a stable and recyclable platform for phenolic analysis.
The developed biosensor is a promising tool for spectrophotometric detection of phenolic compounds.
Further optimization could enhance the reusability and sensitivity of the biosensor.