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Related Experiment Video

Updated: Jun 13, 2026

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients

Published on: July 16, 2018

Lipid vesicle aggregation induced by cooling.

Frank B Howard¹, Ira W Levin

¹Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA. frankhoward@mail.nih.gov <frankhoward@mail.nih.gov>

International Journal of Molecular Sciences

|April 14, 2010

Summary

This summary is machine-generated.

Temperature-dependent lipid vesicle aggregation was observed for dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) vesicles, but not dimyristoyl-sn-glycero-3-phosphocholine (DMPC) vesicles. This reversible aggregation is linked to lipid head group orientation and surface charge.

Keywords:

1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC)1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC)1,2-dipalmitoyl-sn-glycero-3-phosphoserine (DPPS)2-distearoyl-sn-glycero-3-phosphocholine (DSPC)aggregation lipid head groups single shell vesicles

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Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum

Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum

Published on: January 22, 2019

Area of Science:

Biochemistry
Physical Chemistry
Materials Science

Background:

Lipid bilayer fusion is a critical biological process involving complex intermediate steps.
Overcoming electrostatic repulsions and removing water layers are initial requirements for bilayer contact.

Purpose of the Study:

To investigate the reversible aggregation of single-shell lipid vesicles using light scattering.
To understand the factors influencing vesicle adhesion prior to fusion.

Main Methods:

Light scattering measurements were employed to monitor vesicle aggregation.
Experiments were conducted on dimyristoyl-sn-glycero-3-phosphocholine (DMPC), dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) vesicles in aqueous solutions.
Temperature variations were applied to induce and reverse aggregation.

Main Results:

Lowering the temperature induced extensive, reversible aggregation in DPPC and DSPC vesicles.
DMPC vesicles did not exhibit significant aggregation under similar temperature changes.
Aggregation in DPPC and DSPC was attributed to lipid head group orientation and reduced surface charge, while DMPC's failure to aggregate was linked to charged phosphates and buried cholines.

Conclusions:

Lipid head group orientation and surface charge significantly influence vesicle aggregation.
DPPC and DSPC vesicles aggregate reversibly with temperature changes due to specific molecular arrangements.
DMPC vesicles maintain their stability due to distinct surface charge characteristics, preventing aggregation.