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Updated: Jun 13, 2026
Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
Published on: May 1, 2012
Detection of hetero-proteins-mesoporous silica assembly by BRET
Shun-ichi Matsuura1, Tatsuo Tsunoda, Toru Shiomi
1Research Center for Compact Chemical Process, National Institute of Advanced Industrial Science and Technology (AIST), Nigatake 4-2-1, Miyagino-ku, Sendai, 983-8551, Japan. matsuura-shunichi@aist.go.jp
Abstract:
The assembly of a hetero-protein (Renilla reniformis luciferase (Rluc) and a green fluorescence protein (sGFP)) encapsulated in folded-sheet mesoporous material with 7.1 nm pore diameter (FSM7.1), which was used for studying protein-protein interactions in pores of mesoporous silica, has been confirmed by the detection of bioluminescence resonance energy transfers (BRET).
