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Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering
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Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering

Published on: March 1, 2016

Cell attachment-detachment control on temperature-responsive thin surfaces for novel tissue engineering.

Yoshikazu Kumashiro1, Masayuki Yamato, Teruo Okano

  • 1Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University (TWIns), 8-1 Kawadacho, Shinjuku, Tokyo 162-8666, Japan.

Annals of Biomedical Engineering
|April 14, 2010
PubMed
Summary

Temperature-responsive surfaces using poly(N-isopropylacrylamide) enable controlled cell attachment and detachment. This method allows for gentle cell recovery without enzymes, preserving cell sheets and extracellular matrix.

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Published on: October 3, 2014

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Surface Chemistry

Background:

  • Intelligent surfaces offer tunable properties for biological applications.
  • Conventional cell recovery methods often damage cells and extracellular matrix.

Purpose of the Study:

  • To investigate and compare methods for producing temperature-responsive surfaces.
  • To explore their utility in controlled cell attachment and detachment.

Main Methods:

  • Modification of interfaces with poly(N-isopropylacrylamide) and derivatives.
  • Characterization of temperature-dependent hydrophilic/hydrophobic surface changes.
  • Assessment of cell attachment and detachment dynamics on these surfaces.

Main Results:

  • Surfaces exhibit reversible hydrophilic/hydrophobic transitions with temperature.
  • Cell attachment and detachment are modulated by these surface property changes.
  • Cells and extracellular matrix can be recovered intact by temperature reduction.

Conclusions:

  • Temperature-responsive surfaces provide a non-enzymatic method for cell recovery.
  • Surface properties like film thickness and polymer conformation influence cell behavior.
  • Next-generation surfaces aim for even faster cell recovery.