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Related Experiment Video

Updated: Jun 13, 2026

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
10:46

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Published on: September 13, 2022

Using live cells to generate aptamers for cancer study.

Ling Meng1, Kwame Sefah, Dalia Lopez Colon

  • 1Department of Chemistry, Shands Cancer Center and Center for Research at the Bio/Nano Interface, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL, USA.

Methods in Molecular Biology (Clifton, N.J.)
|April 14, 2010
PubMed
Summary

Researchers developed a cell-based method to create aptamers that specifically bind to leukemia cells. This technique identifies cell surface markers for cancer diagnosis and biomarker discovery.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Aptamers are nucleic acid molecules that bind targets with high specificity.
  • Identifying specific cell surface markers is crucial for targeted therapies and diagnostics.
  • Current methods may require prior knowledge of target molecules.

Purpose of the Study:

  • To develop a cell-based aptamer selection strategy for identifying molecular signatures on target cells.
  • To generate and characterize aptamers that specifically recognize leukemia cells.

Main Methods:

  • Utilized cell-based aptamer selection to exploit molecular differences between cell types.
  • Applied the method to identify aptamers targeting leukemia cells.
  • Determined the binding affinity of selected aptamers using equilibrium dissociation constant (Kd).

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Main Results:

  • Generated a panel of aptamers with specific recognition of leukemia cells.
  • Achieved high binding affinity for target cells, with Kd values in the nanomolar-to-picomolar range.
  • Demonstrated a simple, fast, reproducible, and straightforward selection process.

Conclusions:

  • Cell-based aptamer selection is an effective strategy for identifying cell-specific molecular signatures.
  • This method enables aptamer generation without prior knowledge of target markers.
  • Holds significant promise for developing molecular probes for cancer diagnosis and biomarker discovery.