Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 13, 2026

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
12:32

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR

Published on: January 16, 2016

[Development a method of quantitative assay for enterovirus 71].

Jing Xu1, Wen-yu Cui, Shu-xiang Li

  • 1National Vaccine and Serum Institute, 100024, China. xj17b@163.com

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi = Zhonghua Shiyan He Linchuang Bingduxue Zazhi = Chinese Journal of Experimental and Clinical Virology
|April 15, 2010
PubMed
Summary

A new quantitative assay for enterovirus 71 (EV71) was developed using ELISA. This method accurately detects EV71 virus content, crucial for vaccine development and production.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Isolation of functional bacteria guided by PCR-DGGE technology from high temperature petroleum reservoirs].

Huan jing ke xue= Huanjing kexue·2008
Same author

Assessing the spatial extent of breast tumor intrinsic optical contrast using ultrasound and diffuse optical spectroscopy.

Journal of biomedical optics·2008
Same author

alpha4/7-conotoxin Lp1.1 is a novel antagonist of neuronal nicotinic acetylcholine receptors.

Peptides·2008
Same author

[Construction and characterization of avian pathogenic Escherichia coli mutants with iro and/or tsh gene mutation].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology·2008
Same author

[Enhancement of GFP expression by Kozak sequence +4G in HEK293 cells].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology·2008
Same author

Sphingomyelin synthase 2 deficiency attenuates NFkappaB activation.

Arteriosclerosis, thrombosis, and vascular biology·2008

Area of Science:

  • Virology
  • Immunology
  • Biotechnology

Background:

  • Enterovirus 71 (EV71) is a significant human pathogen.
  • Accurate quantification of EV71 is essential for vaccine development and quality control.

Purpose of the Study:

  • To establish a quantitative assay for EV71.
  • To enable precise detection of EV71 virus content during vaccine production.

Main Methods:

  • Development of a double antibody sandwich ELISA.
  • Evaluation of assay sensitivity, accuracy, precision, and specificity.

Main Results:

  • An ELISA method for quantitative EV71 assay was successfully developed.
  • Quantitation limit: 0.23 microg/ml; Quantitation scope: 7.32-0.23 microg/ml (R² = 0.9976).

More Related Videos

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay
11:09

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay

Published on: September 11, 2016

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment
08:34

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment

Published on: March 2, 2016

Related Experiment Videos

Last Updated: Jun 13, 2026

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
12:32

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR

Published on: January 16, 2016

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay
11:09

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay

Published on: September 11, 2016

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment
08:34

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment

Published on: March 2, 2016

  • High accuracy (90%-110% recovery) and precision (CV < 10%) demonstrated.

    • Conclusions:

    • A validated ELISA method for quantitative EV71 assay is available.
    • This assay facilitates rapid and accurate EV71 determination in vaccine development and manufacturing.