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A Time Differential Staining Technique Coupled with Full Bilateral Gill Denervation to Study Ionocytes in Fish
Published on: March 19, 2015
[Changes in protein composition in the goldfish tissues depending on season and temperature of keeping]
Abstract:
Data of SDS-electrophoresis of tissues proteins of the goldfish Carassius auratus netted in winter and summer and kept at different temperatures (+5 degrees C and +20 degrees C) are presented. It has been ascertained that after keeping the "winter" fish at 5 degrees C for 2 weeks quantities of the protein 205 kD in the red muscles and heart and the protein 172 kD in the liver are significantly decreased. A slight decline in contents of the proteins 105 and 45 kD was observed in the white muscles of deacclimated fish. The brain protein composition in the goldfish did not undergo virtual changes under the experimental conditions. It has been shown that the protein spectrums are influenced rather by temperature than by season, since according to SDS-electrophoresis data the protein content of the "summer" goldfish tissues and the protein content of tissues of the "winter" goldfish, which were kept at 5 degrees C no less than for 2 weeks, did not differ.
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