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Updated: Jun 13, 2026

Real-time Live Imaging of T-cell Signaling Complex Formation
Published on: June 23, 2013
T-cell receptor early signalling complex activation in response to interferon-alpha receptor stimulation
Claire N Stevens1, Ann-Marie Simeone, Susan John
1Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E6BT, UK.
Abstract:
Signalling through the IFNalphaR (interferon-alpha receptor) and TCR (T-cell receptor) in Jurkat T lymphocytes results in distinct immune responses. Despite this both receptors elicit ERK (extracellular-signal-regulated kinase)/MAPK (mitogen-activated protein kinase) phosphorylation. Vav and Slp76 are shown to be required for IFNalpha (interferon-alpha)-stimulated ERK activity. These form a subset of proteins which behave identically on stimulation of both receptors. TCR deletion abrogates IFNalphaR-stimulated MAPK activity, whereas the canonical JAK/STAT (Janus kinase/signal transducer and activator of transcription) pathway is unaffected. Thus recruitment of the intact TCR ESC (early signalling complex) is necessary for this downstream MAPK response. Despite using a common ESC, stimulation of the IFNalphaR does not produce the transcriptional response associated with TCR. Up-regulation of the MAPK pathway by IFNalphaR might be important to ensure that the cell responds to only one stimulant.
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