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Updated: Jun 13, 2026

Assay for Adhesion and Agar Invasion in S. cerevisiae
Published on: November 8, 2006
Serine214 of Ras2p plays a role in the feedback regulation of the Ras-cAMP pathway in the yeast Saccharomyces
1Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Nankai District, Tianjin, PR China. baixj2006@gmail.com
Abstract:
In the yeast Saccharomyces cerevisiae, Ras proteins are essential for the Ras-cAMP signaling pathway. A serine to alanine substitution at position 214 in the yeast Ras2p resulted in enhanced sensitivity to heat shock, reduced levels of storage glycogen and enhanced both basal cAMP level and glucose-induced cAMP signal. Further work showed that Ras2(Ala214)p had a higher GTP-binding capability than wild type Ras2p. These results suggested that serine 214 of Ras2p plays a role in the feedback regulation of the Ras-cAMP pathway.
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