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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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Related Experiment Video

Updated: Jun 13, 2026

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants
06:34

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Published on: January 21, 2020

Methodological framework for functional characterization of plant microRNAs.

Ming Chen1, Yijun Meng, Chuanzao Mao

  • 1Department of Bioinformatics, College of Life Sciences, Zhejiang University, Zijingang Campus, Yu Hang Tang Road 388, Hangzhou 310058, PR China. mchen@zju.edu.cn

Journal of Experimental Botany
|April 15, 2010
PubMed
Summary

This review details advanced methods for studying microRNAs (miRNAs), small RNA molecules regulating gene expression. It covers bioinformatics and experimental techniques for plant miRNA analysis, highlighting differences and commonalities with animal research.

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mirMachine: A One-Stop Shop for Plant miRNA Annotation
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mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
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RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

Published on: July 11, 2020

Related Experiment Videos

Last Updated: Jun 13, 2026

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants
06:34

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Published on: January 21, 2020

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
14:41

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

Published on: July 11, 2020

Area of Science:

  • Molecular Biology
  • Genetics
  • Bioinformatics

Background:

  • MicroRNAs (miRNAs) are key regulators of gene expression in animals and plants.
  • Understanding miRNA biogenesis, function, and cloning is crucial for biological research.

Purpose of the Study:

  • To review powerful methodologies for plant microRNA research.
  • To highlight both common and distinct techniques used in plant and animal miRNA studies.

Main Methods:

  • Bioinformatics tools for computational analysis of miRNAs.
  • Experimental approaches for upstream and downstream miRNA analysis.
  • Techniques for qualitative and quantitative miRNA cloning.

Main Results:

  • Significant advancements in miRNA research methodologies have been made.
  • Specific methods like 5' modified RACE are unique to plant miRNA target validation.
  • Numerous shared techniques facilitate comparative studies across species.

Conclusions:

  • This review enhances understanding of diverse miRNA research methodologies.
  • It provides insights into the specific and shared techniques for studying miRNAs in plants and animals.